Journal:

Article Title: Urokinase receptor mediates lung fibroblast attachment and migration toward provisional matrix proteins through interaction with multiple integrins

doi: 10.1152/ajplung.90283.2008

Figure Lengend Snippet: Human lung fibroblasts express urokinase-type plasminogen activator cellular receptor (u-PAR) and integrin β1. A: flow cytometry detection of cell surface expression of u-PAR, integrin β1, and IgG controls on human lung fibroblasts were performed using specific primary antibodies. Cells express u-PAR and integrin β1 with fluorescence intensities of 5 and 50 times higher, respectively, compared with IgG control. B: Western blotting and immunoprecipitation analysis of cell lysates. Shown are the following: fibroblasts attached to vitronectin (20 min) lysed in either Triton X-100 lysis buffer (lane 1) or RIPA buffer (lane 2); human su-PAR (10 ng) control (different lanes from same gel; lanes 3, 5, and 6); human u-PAR from fibroblast lysates immunoprecipitated by a mixture of 2 monoclonal antibodies (American Diagnostica No. 3936 and 3937; lane 4) and detected by a polyclonal antibody against u-PAR (399R); and TX-100 lysates from 293 epithelial cells that do not express u-PAR (lane 7). Both Western and immunoprecipitated (IP) u-PAR migrated at 42 kDa.

Article Snippet: Mouse MoAb and rabbit polyclonal anti-human u-PAR antibodies were from American Diagnostica (Greenwich, CT).

Techniques: Flow Cytometry, Expressing, Fluorescence, Western Blot, Immunoprecipitation, Lysis

Journal: Cell reports

Article Title: Assessing kinetics and recruitment of DNA repair factors using high content screens

doi: 10.1016/j.celrep.2021.110176

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit anti-PAR , Enzo Lab Sciences , Cat# ALX-210-890A-0100.

Techniques: Recombinant, Transfection, Electron Microscopy, Bicinchoninic Acid Protein Assay, Control, Negative Control, esiRNA, Plasmid Preparation, Software, High Content Screening, Microscopy, Cell Culture, Imaging